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Each CSM Single Drop-Out mixture contain all components except for one essential nutrient
Current protocols in molecular biology
Besides these components
The antibiotic enters into sensitive cells by active transport
5 g/L Store at room temperature
LoFlo Medium supplemented with Yeast extract Size:1kg Each CSM Single Drop-Out mixtureFormula g l Glucose 11 KH2PO4 0. 68 Casein Peptone 5 Yeast extract 0. 7 NH4Cl 26. 8 MgCl2 37. 1 CaCl2 1. 1 FeCl3 8. 11 Na2 EDTA 4. 84 ZnSO4 2. 30 H3BO3 1. 11 MnCl2. 4H2O 0. 51 CoCl2 0. 17 CuSO5. 5H2O 0. 15 (NH4)6M07O24. 4H2O 0. 1 Suspend 17. 5 gram powdered medium in 1 litre distilled water Store dry at room temperature Adjust to pH 6. 5
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