TBE Buffer 5X Size:5L 15 Glucose 18 KH2PO4 0

15 Glucose 18 KH2PO4 0

This makes a final concentration of 50mM MOPS

minus Cysteine and without Methionine

Dilute 50 ml MOPS-SDS 20X into 950 ml of deionised water to make 1 litre of MOPS-SDS Running Buffer

In LBM media Mg2 is added to enhance adsorption of phage lambda to the cells

TBE Buffer 5X Size:5L 15 Glucose 18 KH2PO4 05 X sterile stock solution Dilute 100ml TBE Buffer 10X into 900ml deionised water to make 1 litre of TBE Buffer. Final concentration is 89mM Tris base, 89mM Borate and 2mM Na2EDTA. Specifications TRIS 0. 9 M 108. 0 g L Boric acid 0. 9 M 55. 0 g L Na2EDTA 0. 02 M 43. 5 g L Store at room temperature Keep away from light